Journal: Frontiers in Immunology

Article Title: Baicalein links macrophage M2 polarization with reduced synovial inflammation to alleviate gouty arthritis

doi: 10.3389/fimmu.2026.1812532

Figure Lengend Snippet: Schematic diagram of the experimental protocol for establishing a hyperuricemia-induced acute gouty arthritis mouse model and baicalein intervention. Male Balb/c mice were administered Potassium Oxonate (PO) and Yeast Extract via oral gavage from Day 1 to Day 35. Acute gouty arthritis was induced by intra-articular injection of monosodium urate (MSU) crystals into the left ankle joint from Day 29 to Day 35 . Starting from Day 29, all groups received daily oral gavage treatments (including baicalein and saline control). Relevant signs in mice, including body weight, food intake, water intake, urine output, ankle swelling, and gait, were monitored regularly. On Day 35, mice were euthanized, and serum, liver, kidney, and ankle joint tissues were collected for subsequent biochemical, histopathological, and molecular biological analyses. This protocol allows for a comprehensive evaluation of the anti-inflammatory and uricosuric effects of baicalein in a clinically relevant gout model.

Article Snippet: Key reagents for inducing hyperuricemia included Potassium Oxonate and Yeast Extract (both from Shanghai Macklin Biochemical Co., Ltd.).

Techniques: Injection, Saline, Control

Journal: Animal Models and Experimental Medicine

Article Title: Establishment of a novel cynomolgus monkey model of hyperuricemia

doi: 10.1002/ame2.70128

Figure Lengend Snippet: Inhibiting uric acid degradation and decreasing the excretion of the intestine and kidney are conducive to increasing the level of uric acid in the serum of cynomolgus monkeys. (A) Schematic diagram. (B–E) After the upper limit of daily water intake was increased and oxonate potassium and hydrochlorothiazide was administered via fruits and vegetables to cynomolgus monkeys, there were no significant changes in the daily urine output and feces excretion of cynomolgus monkeys. (F–I) The levels of uric acid in the urine and feces of cynomolgus monkeys decreased significantly. Results are represented as mean ± SD (standard deviation); differences between groups were analyzed using the t ‐test (*** p < 0.005, **** p < 0.001, n = 4). (J–M) Significant decrease in 24‐h uric acid excretion in the kidneys and intestines of cynomolgus monkeys. Results are represented as mean ± SD; differences between groups were analyzed using the t ‐test (**** p < 0.001, n = 4). (N) The serum uric acid level of cynomolgus monkeys increased but not significantly.

Article Snippet: Potassium oxonate (S17112, Shanghai Yuanye Bio‐Technology Co., Ltd) and composite feed containing uric acid (U2625‐25G, Sigma), adenine (YB24793, Hunan Yunbang Bio), and yeast powder (MLP0021B, Shanghai Yuanye Bio‐Technology Co., Ltd) were used in the induction phase.

Techniques: Standard Deviation

Journal: Animal Models and Experimental Medicine

Article Title: Establishment of a novel cynomolgus monkey model of hyperuricemia

doi: 10.1002/ame2.70128

Figure Lengend Snippet: Inhibiting uric acid degradation and intestinal and renal excretion, while promoting the synthesis of uric acid, is conducive to increasing the serum uric acid level in cynomolgus monkeys. (A) Schematic diagram. (B–E) After the cynomolgus monkeys were administered oxonate potassium, hydrochlorothiazide, and adenine via fruits and vegetables, there were no significant changes in the daily urine and feces output. (F–I) The levels of uric acid in the urine and feces of cynomolgus monkeys increased significantly. Results are represented as mean ± SD (standard deviation); differences between groups were analyzed using the t ‐test (* p < 0.05, **** p < 0.001, n = 4). (J–M) Significant increase in 24‐h uric acid excretion of the kidneys and intestines of cynomolgus monkeys. Results are represented as mean ± SD; differences between groups were analyzed using the t ‐test (** p < 0.01, *** p < 0.005, n = 4). (N) The serum uric acid level of cynomolgus monkeys significantly increased. Results are represented as mean ± SD; differences between groups were analyzed using the t ‐test (**** p < 0.001, n = 4).

Article Snippet: Potassium oxonate (S17112, Shanghai Yuanye Bio‐Technology Co., Ltd) and composite feed containing uric acid (U2625‐25G, Sigma), adenine (YB24793, Hunan Yunbang Bio), and yeast powder (MLP0021B, Shanghai Yuanye Bio‐Technology Co., Ltd) were used in the induction phase.

Techniques: Standard Deviation